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Avian (H5N1) Infection Resulted in Cardiac and Neuronal Pathway Enrichment. (A) Heatmaps of selected pathways significantly up-regulated in avian influenza at 48hpi, including cardiac conduction and neuronal processes pathways which are enriched with ion transport genes. Gene expression value is presented in Log2FC. (B) The hNECs and/or <t>hBECs</t> infected with H3N2 and H5N1 were subjected to treatment with peruvoside, a modulator of cellular calcium ions, at concentrations of 20, 60 and 100nM. At 100nM, peruvoside significantly reduced the H3N2 viral titre hNECs, and of H5N1 viral titre infected hBECs. Statistical analyses were conducted using 1-Way ANOVA with Dunnett's correction for multiple comparison. * p < 0.05.
Primary Human Bronchial Epithelial Cells Hbecs, supplied by PromoCell, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Avian (H5N1) Infection Resulted in Cardiac and Neuronal Pathway Enrichment. (A) Heatmaps of selected pathways significantly up-regulated in avian influenza at 48hpi, including cardiac conduction and neuronal processes pathways which are enriched with ion transport genes. Gene expression value is presented in Log2FC. (B) The hNECs and/or <t>hBECs</t> infected with H3N2 and H5N1 were subjected to treatment with peruvoside, a modulator of cellular calcium ions, at concentrations of 20, 60 and 100nM. At 100nM, peruvoside significantly reduced the H3N2 viral titre hNECs, and of H5N1 viral titre infected hBECs. Statistical analyses were conducted using 1-Way ANOVA with Dunnett's correction for multiple comparison. * p < 0.05.
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Avian (H5N1) Infection Resulted in Cardiac and Neuronal Pathway Enrichment. (A) Heatmaps of selected pathways significantly up-regulated in avian influenza at 48hpi, including cardiac conduction and neuronal processes pathways which are enriched with ion transport genes. Gene expression value is presented in Log2FC. (B) The hNECs and/or <t>hBECs</t> infected with H3N2 and H5N1 were subjected to treatment with peruvoside, a modulator of cellular calcium ions, at concentrations of 20, 60 and 100nM. At 100nM, peruvoside significantly reduced the H3N2 viral titre hNECs, and of H5N1 viral titre infected hBECs. Statistical analyses were conducted using 1-Way ANOVA with Dunnett's correction for multiple comparison. * p < 0.05.
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Avian (H5N1) Infection Resulted in Cardiac and Neuronal Pathway Enrichment. (A) Heatmaps of selected pathways significantly up-regulated in avian influenza at 48hpi, including cardiac conduction and neuronal processes pathways which are enriched with ion transport genes. Gene expression value is presented in Log2FC. (B) The hNECs and/or <t>hBECs</t> infected with H3N2 and H5N1 were subjected to treatment with peruvoside, a modulator of cellular calcium ions, at concentrations of 20, 60 and 100nM. At 100nM, peruvoside significantly reduced the H3N2 viral titre hNECs, and of H5N1 viral titre infected hBECs. Statistical analyses were conducted using 1-Way ANOVA with Dunnett's correction for multiple comparison. * p < 0.05.
Cell Lines Human Bronchial Epithelial Cells Hbec Atcc, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human bronchial epithelial cells (hbec)  (Lonza)
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Avian (H5N1) Infection Resulted in Cardiac and Neuronal Pathway Enrichment. (A) Heatmaps of selected pathways significantly up-regulated in avian influenza at 48hpi, including cardiac conduction and neuronal processes pathways which are enriched with ion transport genes. Gene expression value is presented in Log2FC. (B) The hNECs and/or <t>hBECs</t> infected with H3N2 and H5N1 were subjected to treatment with peruvoside, a modulator of cellular calcium ions, at concentrations of 20, 60 and 100nM. At 100nM, peruvoside significantly reduced the H3N2 viral titre hNECs, and of H5N1 viral titre infected hBECs. Statistical analyses were conducted using 1-Way ANOVA with Dunnett's correction for multiple comparison. * p < 0.05.
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Avian (H5N1) Infection Resulted in Cardiac and Neuronal Pathway Enrichment. (A) Heatmaps of selected pathways significantly up-regulated in avian influenza at 48hpi, including cardiac conduction and neuronal processes pathways which are enriched with ion transport genes. Gene expression value is presented in Log2FC. (B) The hNECs and/or <t>hBECs</t> infected with H3N2 and H5N1 were subjected to treatment with peruvoside, a modulator of cellular calcium ions, at concentrations of 20, 60 and 100nM. At 100nM, peruvoside significantly reduced the H3N2 viral titre hNECs, and of H5N1 viral titre infected hBECs. Statistical analyses were conducted using 1-Way ANOVA with Dunnett's correction for multiple comparison. * p < 0.05.
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Avian (H5N1) Infection Resulted in Cardiac and Neuronal Pathway Enrichment. (A) Heatmaps of selected pathways significantly up-regulated in avian influenza at 48hpi, including cardiac conduction and neuronal processes pathways which are enriched with ion transport genes. Gene expression value is presented in Log2FC. (B) The hNECs and/or <t>hBECs</t> infected with H3N2 and H5N1 were subjected to treatment with peruvoside, a modulator of cellular calcium ions, at concentrations of 20, 60 and 100nM. At 100nM, peruvoside significantly reduced the H3N2 viral titre hNECs, and of H5N1 viral titre infected hBECs. Statistical analyses were conducted using 1-Way ANOVA with Dunnett's correction for multiple comparison. * p < 0.05.
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human primary bronchial epithelial cells hbecs  (ATCC)
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Avian (H5N1) Infection Resulted in Cardiac and Neuronal Pathway Enrichment. (A) Heatmaps of selected pathways significantly up-regulated in avian influenza at 48hpi, including cardiac conduction and neuronal processes pathways which are enriched with ion transport genes. Gene expression value is presented in Log2FC. (B) The hNECs and/or <t>hBECs</t> infected with H3N2 and H5N1 were subjected to treatment with peruvoside, a modulator of cellular calcium ions, at concentrations of 20, 60 and 100nM. At 100nM, peruvoside significantly reduced the H3N2 viral titre hNECs, and of H5N1 viral titre infected hBECs. Statistical analyses were conducted using 1-Way ANOVA with Dunnett's correction for multiple comparison. * p < 0.05.
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Avian (H5N1) Infection Resulted in Cardiac and Neuronal Pathway Enrichment. (A) Heatmaps of selected pathways significantly up-regulated in avian influenza at 48hpi, including cardiac conduction and neuronal processes pathways which are enriched with ion transport genes. Gene expression value is presented in Log2FC. (B) The hNECs and/or hBECs infected with H3N2 and H5N1 were subjected to treatment with peruvoside, a modulator of cellular calcium ions, at concentrations of 20, 60 and 100nM. At 100nM, peruvoside significantly reduced the H3N2 viral titre hNECs, and of H5N1 viral titre infected hBECs. Statistical analyses were conducted using 1-Way ANOVA with Dunnett's correction for multiple comparison. * p < 0.05.

Journal: Emerging Microbes & Infections

Article Title: Unique immune and other responses of human nasal epithelial cells infected with H5N1 avian influenza virus compared to seasonal human influenza A and B viruses

doi: 10.1080/22221751.2025.2484330

Figure Lengend Snippet: Avian (H5N1) Infection Resulted in Cardiac and Neuronal Pathway Enrichment. (A) Heatmaps of selected pathways significantly up-regulated in avian influenza at 48hpi, including cardiac conduction and neuronal processes pathways which are enriched with ion transport genes. Gene expression value is presented in Log2FC. (B) The hNECs and/or hBECs infected with H3N2 and H5N1 were subjected to treatment with peruvoside, a modulator of cellular calcium ions, at concentrations of 20, 60 and 100nM. At 100nM, peruvoside significantly reduced the H3N2 viral titre hNECs, and of H5N1 viral titre infected hBECs. Statistical analyses were conducted using 1-Way ANOVA with Dunnett's correction for multiple comparison. * p < 0.05.

Article Snippet: Primary human bronchial epithelial cells (hBECs) (PromoCell, Heidelberg, Germany) were expanded in PneumaCult-Ex Plus medium (Stemcell Technologies, Vancouver, Canada) supplemented with 100 IU/mL antibiotic-antimycotic (Gibco-Invitrogen, Waltham, MA, USA), 1 μM A83-01 (Stemcell Technologies, Vancouver, Canada), 5 μM Y-27632 (Stemcell Technologies, Vancouver, Canada) and 3 μM isoprenaline hydrochloride (MedChemExpress, NJ, USA).

Techniques: Infection, Gene Expression, Comparison